Iranian President Hassan Rouhani arrived in Ankara early Wednesday, after lashing out at the United States and Israel on Tuesday for "interference" in Syria.
Rouhani was scheduled to meet with Turkish President Recep Tayyip Erdogan and Russian President Vladimir Putin in the Turkish capital to discuss developments in war-torn Syria. Last November, the three leaders met in Sochi, Russia, to discuss Syria.
"The Americans are against the Syrian government establishing its authority throughout the whole country and are even thinking of a breakup [of the country]," Rouhani said before leaving for Turkey, in a ceremony broadcast live from Tehran's Mehrabad airport.
"There's interference from Zionist forces in Syria which has increased problems. They don't respect Syrian national sovereignty. They bomb areas in Syria. They support terrorists. These are all issues which have increased Syria's problems," Rouhani said.
The meeting of the three leaders brings together Syrian President Bashar Assad's most forceful supporters, Iran and Russia, with one of his strongest opponents, Turkey.
Russia has boosted Assad's military capabilities by bringing in key air support.
Critics have accused Russia of targeting civilians, a charge it has denied.
The U.S. military has primarily limited its intervention in Syria to fighting the Islamic State group, rather than targeting forces allied with the Syrian government.
Rouhani said there is no military solution to the long conflict in Syria, in which 500,000 people have been killed and half the population displaced.
Only dialogue can end the conflict, he said.
Shiite Iran is Assad's main regional ally and has acknowledged having military advisers and volunteers from its elite Revolutionary Guards in Syria.